Semithin Sections (a, B And Immunocytochemistry For Mac
Wallerian degeneration is characterized by breakdown of myelin and axons with subsequent macrophage infiltration and removal of the degenerating nerve components. Proteinases of the matrix. Chemical treatment of the sections before autoradiography may reduce the cbemography. Treatment with 1% hydrogen peroxide for 15 nun reduced it to such an extent that subsequent coating with 5 nm carbon abolished it. Material block stained in this way gave excellent contrast, both for light and electron microscopy.
Semithin Sections (a B And Immunocytochemistry For Macbook Pro
Paraffin sections were cut as described and stained with H&E to assess inflammation, luxol fast blue/cresyl violet (LFB/CV) for demyelination, and Bielschowsky’s silver method for axonal loss. Semithin resin sections were prepared and stained with toluidine blue to further assess demyelination, as described.
. 50 Downloads. Summary The use of butyl-methyl-methacrylate embedding and the application of the silver methenamine (SM) method as a poststaining of the immunoperoxidase-DAB (IP) procedure led to the standardization of several useful methods for the visualization of tissue antigents at the light and electron microscope level.